Objective: A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.
Method: The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).
Result: The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).
Conclusion: The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.