The authors investigated a population group of 660 men and 440 women aged 35-60 years selected in the Skoda works and examined as part of the integrated programme of prevention of chronic non-infectious diseases. The risk profile of subjects associated with the life-style of the population was evaluated with regard to sex, age and education. It was revealed that the blood pressure rises with age and body weight in men and women. With rising body weight rises also the triglyceride and uric acid level and in women also the total cholesterol level. The authors confirmed the relationship between smoking and the thiocyanate blood level. The magnesium level declines with age in both sexes and correlates with the reported alcohol intake. Men and women with higher education smoke less, have a lower body weight and lower triglyceride blood levels. Women have also a lower total cholesterol level. University educated men consume less alcohol, while the reverse is true in university educated women.