Abstract
To investigate the regulatory role of traP (target of RNAIII-activating peptide) in Staphylococcus aureus, we generated traP mutations in the clinical isolates UAMS-1 and USA300. In neither case did mutation of traP affect expression of the accessory gene regulator (agr) or the ability to form a biofilm. We were also unable to confirm that mutation of traP in the prototype 8325-4 laboratory strain RN6390 results in reduced expression of agr, reduced hemolytic activity, or an altered capacity to form a biofilm.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics*
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Biofilms / growth & development*
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Gene Expression Regulation, Bacterial / physiology*
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Mutagenesis*
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RNA-Binding Proteins / genetics*
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / physiology
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Trans-Activators / biosynthesis*
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Trans-Activators / genetics*
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Transcription Factors / genetics*
Substances
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Agr protein, Staphylococcus aureus
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Bacterial Proteins
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MtrB protein, Bacteria
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RNA-Binding Proteins
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Trans-Activators
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Transcription Factors