Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach

J Clin Microbiol. 2007 Aug;45(8):2726-30. doi: 10.1128/JCM.00321-07. Epub 2007 May 30.

Abstract

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Bacterial / genetics
  • Humans
  • Molecular Sequence Data
  • Mycoplasma pneumoniae / genetics
  • Mycoplasma pneumoniae / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Repetitive Sequences, Nucleic Acid / genetics
  • Respiratory System / microbiology*
  • Sensitivity and Specificity
  • Sequence Alignment

Substances

  • DNA, Bacterial