Abstract
Using a yeast one-hybrid method, a transcription factor, OsAP25, which interacts specifically with a GCC box was isolated from rice. The OsAP25 protein contained a conserved ethylene-responsive element binding factor (ERF) domain which shared identity with other reported ERF domains. Phylogenetic analysis showed that OsAP25 could be categorized into class III ERF of the previously characterized ERF proteins on an evolutionary relationship. The semi-quantitative RT-PCR analysis revealed that OsAP25 gene was constitutively expressed in leaves, roots, growing points, flower, bolting stage and grain filling stage. In addition, OsAP25 gene was induced by NaCl, cold, drought, abscisic acid and exogenous ethylene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / metabolism
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Amino Acid Sequence
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Base Sequence
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DNA, Complementary / metabolism*
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Molecular Sequence Data
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Oryza / metabolism*
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Phylogeny
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Plant Leaves / metabolism
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Plant Proteins / chemistry*
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plant Roots / metabolism
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Two-Hybrid System Techniques
Substances
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DNA, Complementary
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Plant Proteins
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Receptors, Cell Surface
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Transcription Factors
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ethylene receptors, plant
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Abscisic Acid