Abstract
Group A rotaviruses are the main cause of acute gastroenteritis in children throughout the world. The two outer capsid proteins, VP4 and VP7, define the P and G genotypes, respectively. Rotaviruses with P[8]G1, P[4]G2, P[8]G3 and P[8]G4 genotypes are predominant in infecting humans and the G9 genotype is emerging in most continents as the fifth most common G type worldwide. The inner capsid protein VP6 is responsible for subgroup (SG) specificities, allowing classification of rotaviruses into SG I, SG II, SG I+II and SG non-I-non-II. The non-structural protein 4 (NSP4) encoded by segment 10 has a role in viral morphogenesis and five genetic groups have been described, NSP4 genotypes A-E. The aim of this investigation was to characterize the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children. Thirty rotavirus strains were submitted to RT-PCR followed by sequencing and phylogenetic analysis. Among the different G and P genotype combinations, two distinct genetic groups could be recognized for the NSP4 gene. Twenty-eight clustered with NSP4 genotype B. The two P[4]G2 strains fell into NSP4 genotype A and clustered distinctly, with a 100 % bootstrap value. The strains distinguished within a group were closely related to each other at the nucleotide and amino acid levels. A phylogenetic tree was constructed for the VP6 gene including the human strains RMC100, E210, Wa, US1205 and 1076, and the animal strains Gott, NCDV, SA-11, FI-14 and EW. This is the first report on Brazilian rotavirus strains describing NSP4 genotype A strains associated with VP6 SG I, and NSP4 genotype B strains associated with VP6 SG II.
MeSH terms
-
Amino Acid Sequence
-
Antigens, Viral / genetics*
-
Brazil
-
Capsid Proteins / genetics*
-
Child
-
Child, Hospitalized
-
Cluster Analysis
-
Genotype
-
Glycoproteins / genetics*
-
Humans
-
Molecular Epidemiology
-
Molecular Sequence Data
-
Phylogeny
-
RNA, Viral / genetics
-
Reverse Transcriptase Polymerase Chain Reaction
-
Rotavirus / classification*
-
Rotavirus / genetics*
-
Rotavirus / isolation & purification
-
Rotavirus Infections / virology*
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Sequence Homology, Nucleic Acid
-
Toxins, Biological / genetics*
-
Viral Nonstructural Proteins / genetics*
Substances
-
Antigens, Viral
-
Capsid Proteins
-
Glycoproteins
-
NS28 protein, rotavirus
-
RNA, Viral
-
Toxins, Biological
-
VP6 protein, Rotavirus
-
Viral Nonstructural Proteins
Associated data
-
GENBANK/DQ498142
-
GENBANK/DQ498143
-
GENBANK/DQ498144
-
GENBANK/DQ498145
-
GENBANK/DQ498146
-
GENBANK/DQ498147
-
GENBANK/DQ498148
-
GENBANK/DQ498149
-
GENBANK/DQ498150
-
GENBANK/DQ498151
-
GENBANK/DQ498152
-
GENBANK/DQ498153
-
GENBANK/DQ498154
-
GENBANK/DQ498155
-
GENBANK/DQ498156
-
GENBANK/DQ498157
-
GENBANK/DQ498158
-
GENBANK/DQ498159
-
GENBANK/DQ498160
-
GENBANK/DQ498161
-
GENBANK/DQ498162
-
GENBANK/DQ498163
-
GENBANK/DQ498164
-
GENBANK/DQ498165
-
GENBANK/DQ498166
-
GENBANK/DQ498167
-
GENBANK/DQ498168
-
GENBANK/DQ498169
-
GENBANK/DQ498170
-
GENBANK/DQ498171
-
GENBANK/DQ498172
-
GENBANK/DQ498173
-
GENBANK/DQ498174
-
GENBANK/DQ498175
-
GENBANK/DQ498176
-
GENBANK/DQ498177
-
GENBANK/DQ498178
-
GENBANK/DQ498179
-
GENBANK/DQ498180
-
GENBANK/DQ498181
-
GENBANK/DQ498182
-
GENBANK/DQ498183
-
GENBANK/DQ498184
-
GENBANK/DQ498185
-
GENBANK/DQ498186
-
GENBANK/DQ498187
-
GENBANK/DQ498188
-
GENBANK/DQ498189
-
GENBANK/DQ498190
-
GENBANK/DQ498191
-
GENBANK/DQ498192
-
GENBANK/DQ498193
-
GENBANK/DQ498194
-
GENBANK/DQ498195
-
GENBANK/DQ498196
-
GENBANK/DQ498197
-
GENBANK/DQ498198
-
GENBANK/DQ498199
-
GENBANK/DQ498200
-
GENBANK/DQ498201