Abstract
Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER. ORMOCER related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER is shown.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biocompatible Materials / chemistry
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Biocompatible Materials / pharmacology
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Biocompatible Materials / radiation effects
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CHO Cells
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Cell Adhesion / drug effects
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Cell Adhesion / physiology
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Cell Communication / drug effects
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Cell Communication / genetics
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Cell Line, Tumor
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Cell Proliferation / drug effects*
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Ceramics / chemistry
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Ceramics / pharmacology*
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Ceramics / radiation effects
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Computer-Aided Design / trends
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Cricetinae
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Cricetulus
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DNA Damage / drug effects
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DNA Damage / physiology
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Gap Junctions / drug effects
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Gap Junctions / physiology
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Guided Tissue Regeneration / instrumentation
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Guided Tissue Regeneration / methods*
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Humans
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Lasers
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Materials Testing / methods
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Organically Modified Ceramics
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Photochemistry / methods*
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Polymers / chemistry
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Polymers / pharmacology
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Polymers / radiation effects
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Silanes / chemistry
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Silanes / pharmacology*
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Silanes / radiation effects
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Tissue Engineering / instrumentation
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Tissue Engineering / methods*
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Tissue Scaffolds*
Substances
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Biocompatible Materials
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Organically Modified Ceramics
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Polymers
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Silanes