Analysis of coenzyme Q in human blood and tissues

Mitochondrion. 2007 Jun:7 Suppl:S89-93. doi: 10.1016/j.mito.2007.04.002. Epub 2007 Apr 13.

Abstract

The major coenzyme Q species in humans is the decaprenyl quinoid derivative coenzyme Q10 (CoQ10), and its measurement is somewhat challenging owing to its hydrophobicity and tendency to be oxidized. There are three major methods which are suited for analysis of CoQ10: HPLC-coupled UV or electrochemical detection, and tandem mass spectrometry. The techniques are discussed, and results of these applications to determine CoQ10 concentrations in various human fluids and tissues are summarized.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Chromatography, High Pressure Liquid / methods
  • Clinical Trials as Topic
  • Coenzymes / biosynthesis
  • Coenzymes / blood
  • Electrochemistry / methods
  • Erythrocytes / metabolism
  • Gene Expression Regulation, Enzymologic*
  • Humans
  • Mass Spectrometry / methods
  • Middle Aged
  • Oxygen / metabolism
  • Spectrophotometry, Ultraviolet
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / biosynthesis*
  • Ubiquinone / blood*

Substances

  • Coenzymes
  • Ubiquinone
  • coenzyme Q10
  • Oxygen