Screening of exopolysaccharide-producing Lactobacillus and Bifidobacterium strains isolated from the human intestinal microbiota

Appl Environ Microbiol. 2007 Jul;73(13):4385-8. doi: 10.1128/AEM.02470-06. Epub 2007 May 4.

Abstract

Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Bifidobacterium / classification
  • Bifidobacterium / genetics
  • Bifidobacterium / isolation & purification*
  • Bifidobacterium / metabolism*
  • Ecosystem
  • Genes, Bacterial
  • Glycosyltransferases / genetics
  • Glycosyltransferases / metabolism
  • Humans
  • Intestines / microbiology*
  • Lactobacillus / classification
  • Lactobacillus / genetics
  • Lactobacillus / isolation & purification*
  • Lactobacillus / metabolism*
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • Polymerase Chain Reaction
  • Polysaccharides, Bacterial / biosynthesis*
  • Polysaccharides, Bacterial / genetics
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • Polysaccharides, Bacterial
  • Glycosyltransferases