Bacillus qingdaonensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a crude sea-salt sample collected near Qingdao in eastern China

Int J Syst Evol Microbiol. 2007 May;57(Pt 5):1143-1147. doi: 10.1099/ijs.0.64668-0.

Abstract

A moderately haloalkaliphilic, Gram-positive bacterium, designated as strain CM1(T), was isolated from a crude sea-salt sample collected near Qingdao in eastern China. Strain CM1(T) was found to grow optimally at 37 degrees C and pH 9.0. It was shown to be aerobic, rod-shaped and capable of growth at salinities of 2.5-20 % (w/v) NaCl (optimum, 12 %). The genomic DNA G+C content was about 48 mol%. The major cellular fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) and the major isoprenoid quinones were MK-7(H(2)) and MK-6(H(2)). Phylogenetic analyses based on 16S rRNA gene sequences revealed that CM1(T) is a member of the genus Bacillus and has less than 95.2 % gene sequence similarity to the most closely related strain, Bacillus salarius BH169(T). Its DNA-DNA reassociation value with respect to B. salarius BH169(T) was 35.4 %. On the basis of phenotypic and molecular properties, strain CM1(T) represents a novel Bacillus species, for which the name Bacillus qingdaonensis sp. nov. is proposed. The type strain is CM1(T) (=CGMCC 1.6134(T)=JCM 14087(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / chemistry
  • Bacillus / classification*
  • Bacillus / isolation & purification*
  • Bacillus / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Environmental Microbiology*
  • Fatty Acids / analysis
  • Genes, rRNA
  • Hydrogen-Ion Concentration
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Quinones / analysis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Salts / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Salts

Associated data

  • GENBANK/DQ115802