Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp. globosum and its use for expression vector construction

Plasmid. 2007 Sep;58(2):140-7. doi: 10.1016/j.plasmid.2007.02.004. Epub 2007 Apr 30.

Abstract

Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Pairing
  • Base Sequence
  • Bifidobacterium / genetics*
  • Chromosomes, Bacterial
  • Conserved Sequence
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Genetic Vectors / genetics*
  • Glucuronidase / analysis
  • Molecular Sequence Data
  • Open Reading Frames
  • Physical Chromosome Mapping
  • Plasmids / genetics*
  • Promoter Regions, Genetic
  • RNA, Bacterial / genetics
  • RNA, Ribosomal / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • RNA, Bacterial
  • RNA, Ribosomal
  • Glucuronidase