Abstract
Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Base Pairing
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Base Sequence
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Bifidobacterium / genetics*
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Chromosomes, Bacterial
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Conserved Sequence
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Genetic Vectors / genetics*
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Glucuronidase / analysis
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Molecular Sequence Data
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Open Reading Frames
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Physical Chromosome Mapping
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Plasmids / genetics*
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Promoter Regions, Genetic
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RNA, Bacterial / genetics
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RNA, Ribosomal / genetics
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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DNA, Bacterial
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RNA, Bacterial
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RNA, Ribosomal
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Glucuronidase