Abstract
Triphlorethol-A, phlorotannin found in Ecklonia cava, induced heme oxygenase-1 (HO-1) expression at mRNA and protein levels, leading to increased HO-1 activity. Transcription factor NF-E2 related factor 2 (Nrf2) regulates antioxidant response element (ARE) of phase 2 detoxifying and antioxidant enzymes. Triphlorethol-A increased nuclear translocation, ARE binding, and transcriptional activity of Nrf2. Triphlorethol-A exhibited activation of ERK and U0126, inhibitor of ERK kinase, suppressed triphlorethol-A induced activation of Nrf2, finally decreased HO-1 protein level. Taken together, these data suggest that triphlorethol-A augments cellular antioxidant defense capacity through induction of HO-1 via ERK-Nrf2-ARE signaling pathway, thereby protecting cells from oxidative stress.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CHO Cells
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Cell Death / drug effects
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Cells, Cultured
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Cricetinae
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Cricetulus
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Enzyme Activation / drug effects
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Enzyme Induction / drug effects
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Extracellular Signal-Regulated MAP Kinases / metabolism*
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Heme Oxygenase-1 / biosynthesis*
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Heme Oxygenase-1 / genetics
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Models, Biological
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NF-E2-Related Factor 2 / metabolism*
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Oxidative Stress / drug effects
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Phloroglucinol / analogs & derivatives*
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Phloroglucinol / chemistry
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Phloroglucinol / pharmacology
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Phosphorylation / drug effects
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Protein Transport / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Transcription, Genetic / drug effects
Substances
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NF-E2-Related Factor 2
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RNA, Messenger
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triphlorethol-A
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Phloroglucinol
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Heme Oxygenase-1
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Extracellular Signal-Regulated MAP Kinases