Acetone extract from aerial parts of Sideritis ozturkii Aytaç & Aksoy and its fractions were investigated for its in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity assessment, carrageenan-induced hind paw edema and for the antinociceptive activity, p-benzoquinone-induced abdominal constriction tests were used. Acetone extract of the plant and its phenolic fraction were found to possess significant inhibitory activity on these in vivo models in mice. Ozturkoside A (chrysoeriol 7-O-[2'''-O-caffeoyl-6'''-O-acetyl-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside]); ozturkoside B (chrysoeriol 7-O-[2'''-O-caffeoyl-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside]); and ozturkoside C (chrysoeriol 7-O-[2'''-O-p-coumaroyl-6'''-O-acetyl-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranoside]) were isolated from the active phenolic fraction. The structures of isolated compounds were elucidated by spectroscopic techniques (UV, IR, 1D- and 2D-NMR, MS). Ozturkoside C showed notable antinociceptive and anti-inflammatory activities without inducing any apparent acute toxicity or gastric damage. Although the activity of ozturkosides A and B were found insignificant in statistical analysis, some inhibitory effect was observed. Accordingly, it is suggested that these components in phenolic fraction might possibly share the antinociceptive and anti-inflammatory activities together.