In situ hybridization for Lawsonia intracellularis--specific 16s rRNA sequence in paraffin-embedded tissue using a digoxigenin-labeled oligonucleotide probe

J Vet Diagn Invest. 2007 May;19(3):282-5. doi: 10.1177/104063870701900309.

Abstract

An in situ hybridization (ISH) procedure with a digoxigenin-labeled oligonucleotide probe for detection of Lawsonia intracellularis in paraffin-embedded tissue is described. This technique recognized 71% of PCR-positive cases and was thus superior to Warthin-Starry silver stain, which only detected 41%. The presented ISH is of comparable sensitivity to previously published immunohistochemical assays and is recommended for laboratories wishing to diagnose L. intracellularis infections in tissue sections but without access to antibodies.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • DNA, Bacterial / genetics
  • Desulfovibrionaceae Infections / diagnosis
  • Desulfovibrionaceae Infections / microbiology
  • Desulfovibrionaceae Infections / veterinary*
  • Digoxigenin
  • In Situ Hybridization / methods
  • In Situ Hybridization / veterinary*
  • Intestinal Diseases / diagnosis
  • Intestinal Diseases / microbiology
  • Intestinal Diseases / veterinary*
  • Lawsonia Bacteria / genetics
  • Lawsonia Bacteria / isolation & purification*
  • Oligonucleotide Probes / genetics*
  • Paraffin Embedding
  • RNA, Ribosomal, 16S / genetics*
  • Sensitivity and Specificity
  • Swine
  • Swine Diseases / diagnosis
  • Swine Diseases / microbiology*

Substances

  • DNA, Bacterial
  • Oligonucleotide Probes
  • RNA, Ribosomal, 16S
  • Digoxigenin