The fluoride cleavable 2-(cyanoethoxy)methyl (CEM) group as reversible 3'-O-terminator for DNA sequencing-by-synthesis - synthesis, incorporation, and cleavage

Nucleosides Nucleotides Nucleic Acids. 2007;26(3):271-5. doi: 10.1080/15257770701257301.

Abstract

A new and promising sequencing technology called sequencing-by-synthesis (SBS) enables fast determination of DNA sequences. 2'-Deoxynucleotides containing the (2-cyanoethoxy)methyl (CEM) group at the 3'-O-position are potential reversible terminators for the SBS technology. Herein we describe the synthesis, the incorporation by several polymerases, and the cleavage of this 3'-O-blocking group using 3'-O-CEM-thymidinyl-5'-O-triphosphate 7 as an example.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylation
  • Base Sequence
  • Chemistry / methods*
  • Chromatography, High Pressure Liquid
  • DNA / chemistry
  • Fluorescent Dyes / pharmacology
  • Models, Chemical
  • Molecular Sequence Data
  • Nucleotides / chemistry
  • Phosphates / chemical synthesis*
  • Phosphates / chemistry
  • Sequence Analysis, DNA / instrumentation*
  • Sequence Analysis, DNA / methods
  • Templates, Genetic

Substances

  • Fluorescent Dyes
  • Nucleotides
  • Phosphates
  • DNA