Abstract
A new and promising sequencing technology called sequencing-by-synthesis (SBS) enables fast determination of DNA sequences. 2'-Deoxynucleotides containing the (2-cyanoethoxy)methyl (CEM) group at the 3'-O-position are potential reversible terminators for the SBS technology. Herein we describe the synthesis, the incorporation by several polymerases, and the cleavage of this 3'-O-blocking group using 3'-O-CEM-thymidinyl-5'-O-triphosphate 7 as an example.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkylation
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Base Sequence
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Chemistry / methods*
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Chromatography, High Pressure Liquid
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DNA / chemistry
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Fluorescent Dyes / pharmacology
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Models, Chemical
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Molecular Sequence Data
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Nucleotides / chemistry
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Phosphates / chemical synthesis*
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Phosphates / chemistry
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Sequence Analysis, DNA / instrumentation*
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Sequence Analysis, DNA / methods
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Templates, Genetic
Substances
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Fluorescent Dyes
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Nucleotides
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Phosphates
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DNA