During a 5-month summer period, samples of tertiary treated wastewater flowing in an extensive distribution system composed of storage tanks and pipes were collected at 2-week intervals, from 21 different sampling points, including the exit from the wastewater treatment plant (WWTP). The WWTP producing this effluent treated one of the most popular European tourist resorts on the north coast of the island of Crete, at the southernmost point of Greece. The average summer equivalent population treated by the plant exceeded 50,000, falling to under 5,000 in the winter. The samples were analyzed for total coliforms number (TC in cfu / 100 mL) and residual chlorine concentration (RCHL in mg/L). In almost 60% of the samples the TC number was so large that it could be safe to suggest that the WWTP and distribution system failed to reach the EPA standards for wastewater reuse for tree irrigation (faecal coliforms < or = 200 cfu/100 mL and RCHL < 1 mg/L) due mainly to inflow and septage handling, above designed limits. The licensing operational and reuse standards were set in TC < or = 500 cfu/100 mL, where 76% of the analyzed samples recorded larger values. It was also estimated that in 14% of the samples the wastewater quality failed to reach even the less strict WHO standards of FC < or = 1.000 cfu/100 mL. When chlorination was properly adjusted the effluent quality was improved (last three sets of samplings for each collection point), helped by the WWTP inflow decrease. As a result the values recorded in all tanks and collectors were also improved. However, even then, the required licensing standards were randomly and barely achieved. This suggests that specific wastewater quality standard at the exit of a WWTP do not guarantee that the wastewater will still retain these characteristics at its final application point.