The presented study aimed to explore the relationships between ambient ozone (O3) and tree defoliation, specific diversity, and abundance of soil microarthropods, stream macroinvertebrates, and small mammals (mainly rodents) in order to test the hypothesis that changes in the considered objects of the forest ecosystem could be related to changes in ambient O3, concentration of which is below critical level. The observations were carried out from 1994 at three integrated monitoring stations. The obtained data revealed that only peak O3 concentrations (from 125-215 microg x m(-3)) had significant effect on changes in the considered components of forest biota.