The objective of this study was to create a three-dimensional mathematical model of a human masticatory system, including the mandible, the dentition and the temporomandibular joints. Object of research was one 20 year old dead man. The research was approved by Committee of bioethics (Kaunas University of Medicine). Required extent of computed tomography scanning and required high amount and high resolution of images increased X-ray radiation for the object and made this research impossible to perform on alive human. Spiral computed tomography scanning was performed to achieve two-dimensional images, necessary for creating three-dimensional model. The 3D modeling was done using the "Image pro plus" and "Imageware"software. A three-dimensional physiological (normal) model of a human masticatory system, simulating the mandible, the dentition and the temporomandibular joints was generated. This model system will be used subsequently in stress analysis comparison for the physiological and pathological systems after improvement of its physical properties. We suggest that computer simulation is a promising way to study musculoskeletal biomechanics of masticatory system.