Abstract
The genes encoding Pasteurella multocida lipoprotein E (PlpE) and lipoprotein B (PlpB) were cloned from P. multocida strain X-73 (serotype A:1) and expressed in Escherichia coli. The protective immunity conferred by recombinant PlpE (r-PlpE) and PlpB (r-PlpB) on mice and chickens was evaluated. The results showed that mice immunized with 10microg of purified r-PlpE were protected (80-100% survival rate) against challenge infection with 10 or 20 LD(50) of P. multocida strains X-73 (serotype A:1), P-1059 (serotype A:3) and P-1662 (serotype A:4). In contrast, mice immunized with r-PlpB were not protected. Chickens immunized with 100microg of purified r-PlpE were protected (63-100% survival rate) against lethal challenge infection with strains X-73 and P-1662, whereas those immunized with r-PlpB were not. Sequence analyses showed that PlpE from different strains of P. multocida exhibited 90.8-100% sequence identity to each other, suggesting that PlpE might serve as a cross-protective antigen. This is the first report of a recombinant P. multocida antigen that confers cross protection on animals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Apolipoproteins B / genetics
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Apolipoproteins B / immunology
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Apolipoproteins E / genetics
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Apolipoproteins E / immunology*
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / immunology*
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Bacterial Outer Membrane Proteins / isolation & purification
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Bacterial Vaccines / immunology*
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Chickens
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Cloning, Molecular
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Disease Models, Animal
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Escherichia coli / genetics
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Gene Expression
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Lipoproteins / genetics
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Lipoproteins / immunology*
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Lipoproteins / isolation & purification
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Pasteurella Infections / immunology*
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Pasteurella Infections / prevention & control
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Pasteurella multocida / immunology*
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Sequence Alignment
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Sequence Analysis, DNA
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Specific Pathogen-Free Organisms
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Survival Analysis
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Vaccines, Subunit / genetics
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Vaccines, Subunit / immunology
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Vaccines, Synthetic / isolation & purification
Substances
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Apolipoproteins B
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Apolipoproteins E
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Bacterial Outer Membrane Proteins
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Bacterial Vaccines
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DNA, Bacterial
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Lipoproteins
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Vaccines, Subunit
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Vaccines, Synthetic
Associated data
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GENBANK/EF219452
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GENBANK/EF219453
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GENBANK/EF219454
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GENBANK/EF219455
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GENBANK/EF219456
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GENBANK/EF219457