New insights into the Enterococcus faecalis CroRS two-component system obtained using a differential-display random arbitrarily primed PCR approach

Appl Environ Microbiol. 2007 Jun;73(11):3738-41. doi: 10.1128/AEM.00390-07. Epub 2007 Apr 13.

Abstract

Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Carrier Proteins / genetics
  • DNA, Bacterial / metabolism
  • DNA-Binding Proteins / metabolism
  • Electrophoretic Mobility Shift Assay
  • Enterococcus faecalis / genetics*
  • Gene Expression Regulation, Bacterial*
  • Operon / genetics
  • Promoter Regions, Genetic
  • Protein Binding
  • Random Amplified Polymorphic DNA Technique / methods*
  • Signal Transduction / genetics
  • Transcription Factors / metabolism*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Transcription Factors
  • glutamine transport proteins