Objective: To establish the Chinese Hamster Ovary (CHO) expression system for the human glutathione-S-transferase (GST) A1.
Methods: CHO cells was transfected with recombinant vector pDNA3.1- GSTA1 by lipofectamineTM 2000. The expression of selected CHO-GSTA1 in G418-resistant clones were determined by PCR, RT-PCR and Western blot. CHO-GSTA1 high-expressed clone was cultured to collect supernatant and activity was assayed by U-2001 ultraviolet spectrophotometer.
Results: CHO-GSTA1 was obtained by PCR, RT-PCR and Western blot. The expressed protein of GSTA1 had biological activity, it was 35 mmol/(mg min pro).
Conclusion: The CHO-GSTA1 was constructed and for research purification protein and candidate strains of detoxicitic mechanism of chemical toxins and carcinogenesis.