Abstract
The Caulobacter crescentus DNA adenine methyltransferase CcrM and its homologs in the alpha-Proteobacteria are essential for viability. CcrM is 34% identical to the yhdJ gene products of Escherichia coli and Salmonella enterica. This study provides evidence that the E. coli yhdJ gene encodes a DNA adenine methyltransferase. In contrast to an earlier report, however, we show that yhdJ is not an essential gene in either E. coli or S. enterica.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Base Sequence
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Binding Sites / genetics
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Catalytic Domain / genetics
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DNA Methylation
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DNA Modification Methylases / genetics
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DNA Modification Methylases / metabolism*
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Deoxyribonucleases, Type II Site-Specific / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Plasmids / genetics
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Plasmids / metabolism
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Salmonella enterica / enzymology*
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Salmonella enterica / genetics
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics
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Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism*
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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DNA Modification Methylases
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Site-Specific DNA-Methyltransferase (Adenine-Specific)
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YhdJ protein, E coli
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ATGCAT-specific type II deoxyribonucleases
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Deoxyribonucleases, Type II Site-Specific