Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease

J Virol Methods. 2007 Jul;143(1):81-5. doi: 10.1016/j.jviromet.2007.02.009. Epub 2007 Mar 29.

Abstract

An automated one-step real-time reverse transcription polymerase chain reaction (rRT-PCR) protocol was optimised and evaluated for the routine diagnosis of foot-and-mouth disease (FMD). Parallel testing of RNA samples (n=257) indicated that this assay has a diagnostic sensitivity at least equivalent to the automated two-step rRT-PCR protocol previously used for the laboratory detection of FMD virus (FMDV). This more rapid and economical one-step protocol will play a key role in contingency planning for any future outbreaks of FMD in the United Kingdom (UK).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cattle Diseases / diagnosis*
  • Foot-and-Mouth Disease / diagnosis*
  • Foot-and-Mouth Disease Virus / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • United Kingdom