Abstract
Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity and anticancer properties in JB6 P (+) mouse epidermal cells, human lung and leukemia cells. Deerberries contain potent free radical scavenging activities. Pretreatment of JB6 P (+) mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) induced by either 12- O-tetradecanoylphorbol 13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK 1/2, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL-60 cancer cells and human lung epithelial cancer A549 cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-kappaB activity, inhibition of HL-60 cells and cancer A549 cells proliferation and induction of apoptotic in human leukemia HL-60 cancer cells may be mediated through the ERKs and MEK 1/2 signal pathway.
MeSH terms
-
Animals
-
Antineoplastic Agents, Phytogenic / chemistry
-
Antineoplastic Agents, Phytogenic / isolation & purification
-
Antineoplastic Agents, Phytogenic / pharmacology*
-
Antioxidants / chemistry
-
Antioxidants / isolation & purification
-
Antioxidants / pharmacology*
-
Apoptosis / drug effects
-
Cell Line, Tumor
-
Cell Proliferation / drug effects
-
Chromatography, High Pressure Liquid
-
Enzyme Activation / drug effects
-
Enzyme Activation / radiation effects
-
Flavonoids / chemistry
-
Flavonoids / isolation & purification
-
Fruit / chemistry
-
Humans
-
Leukemia / enzymology
-
Leukemia / metabolism
-
Lung Neoplasms / enzymology
-
Lung Neoplasms / metabolism
-
Mice
-
Mitogen-Activated Protein Kinases / antagonists & inhibitors
-
Mitogen-Activated Protein Kinases / metabolism
-
NF-kappa B / antagonists & inhibitors
-
Phosphorylation / drug effects
-
Phosphorylation / radiation effects
-
Plant Extracts / chemistry
-
Plant Extracts / isolation & purification
-
Plant Extracts / pharmacology*
-
Reactive Oxygen Species / metabolism
-
Tetradecanoylphorbol Acetate / pharmacology
-
Transcription Factor AP-1 / antagonists & inhibitors
-
Ultraviolet Rays
-
Vaccinium / chemistry*
Substances
-
Antineoplastic Agents, Phytogenic
-
Antioxidants
-
Flavonoids
-
NF-kappa B
-
Plant Extracts
-
Reactive Oxygen Species
-
Transcription Factor AP-1
-
Vaccinium stamineum fruit extract
-
Mitogen-Activated Protein Kinases
-
Tetradecanoylphorbol Acetate