Abstract
iASPP is an inhibitory member of ASPP (apoptosis stimulating protein of p53, or Ankyrin repeats, SH3 domain and proline-rich region contain Protein) family. As reported previously, it at least includes two isoforms, one is iASPP/RAI (351 amino acids, aa) and the other is iASPP (828 aa).Here, we identified a novel open reading frame of human iASPP, which encodes a 407 aa protein and highly matches with the C terminus of iASPP (828 aa, CAI60219). Hereafter, iASPP (407 aa) will be referred to as iASPP-SV (iASPP splice variant). In further study, we found that iASPP-SV is a nuclear protein, and is capable of binding to p53 in vivo. Moreover, overexpression of iASPP-SV can inhibit the transcriptional activity of p53 on the promoters of both Bax and p21.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line, Tumor
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Cyclin-Dependent Kinase Inhibitor p21 / metabolism
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Gene Expression Regulation*
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Molecular Sequence Data
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Promoter Regions, Genetic*
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Protein Binding
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Repressor Proteins
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Tumor Suppressor Protein p53 / metabolism*
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bcl-2-Associated X Protein / metabolism
Substances
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Cyclin-Dependent Kinase Inhibitor p21
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Intracellular Signaling Peptides and Proteins
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PPP1R13L protein, human
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Protein Isoforms
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Repressor Proteins
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein