This study determined the biotypes of group A streptococci (GAS) isolated from 66 pharyngeal and 62 skin and soft-tissue infections. Among all GAS isolates tested, the most common biotypes were 1 and 3, irrespective of the isolation source and the severity of clinical symptoms. However, compared with the pharyngeal group, a more heterogeneous distribution of biotypes was observed among the cutaneous group of isolates, including seven isolates that were non-typeable but had an identical biotype pattern, suggesting that they may represent a new biotype.