Adherence to HeLa cells, typing by killer toxins and susceptibility to antifungal agents of Candida dubliniensis strains

Braz Oral Res. 2007 Jan-Mar;21(1):87-91. doi: 10.1590/s1806-83242007000100015.

Abstract

The aim of this study was to evaluate the adherence capability to HeLa cells, the susceptibility to killer toxins and the in vitro susceptibility to antifungal agents (eTest method--AB Biodisk, Solna, Sweden) of 9 Candida dubliniensis isolates recovered from HIV+ and AIDS patients. The adherence test was strongly positive for strain ATCC 777 and positive for all other strains. Typing by killer toxins revealed two different biotypes among the 9 isolates studied: 888 and 688. Only biotype 688 (ATCC 777) was susceptible to the K2 toxin. There was a significant inverse correlation between adherence and killer toxin susceptibility (r = -0.8525 - p = 0.0035). No strains presented resistance to fluconazole, itraconazole, ketoconazole, voriconazole, flucytosine or amphotericin-B. With the exception of ATCC 777, all the other isolates presented similar behavior.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / microbiology
  • Antifungal Agents / pharmacology*
  • Candida / classification
  • Candida / drug effects*
  • Candida / physiology
  • Cell Adhesion
  • Drug Resistance, Microbial*
  • Fungal Proteins / analysis*
  • HIV Infections / microbiology
  • HeLa Cells / microbiology
  • Humans
  • Killer Factors, Yeast
  • Microbial Sensitivity Tests
  • Mycological Typing Techniques
  • Proteins / analysis
  • Proteins / pharmacology

Substances

  • Antifungal Agents
  • Fungal Proteins
  • Killer Factors, Yeast
  • Proteins