The study evaluates the body fluid exposure among health care workers (HCW) at IRCCS Policlinico San Matteo of Pavia from the 1994 through 2003 years. Our data showed that all HCW for less than 5 years of employment, nurses, mid-wifes and surgeons are the most exposed occupational groups. Some variables were compared with a previous study performed from 1983 to 1994 at the same Institution. The study documented 1 case of seroconversion to hepatitis C virus among a physician after percutaneous exposure. In order to reduce the body fluid exposure among the HCW, the authors underline the importance of the administrative and educational interventions carried out in collaboration with the Infection Control Committee and Occupational Medicine Service.