Purpose: This study was conducted to investigate whether thrombus formation at the surface of guide wires occurs, and--if so--whether this can be suppressed or prevented through incorporation of heparin in the surface coating.
Materials and methods: Five guide wire models were examined; three had a polymeric hydrophilic surface coating (90/10 guide wire), which was either heparin-free, impregnated with sodium-heparin (Na-hep), or impregnated with benzalkonium heparin (BAK-hep). The other two guide wires had a coating of polytetrafluoroethylene (PTFE), either without heparin, or impregnated with BAK-hep. Release of heparin, exposure of heparin at the surface of the guide wires, thrombogenicity (under static and flow conditions) and their propensity to attract blood platelets were investigated.
Results: The guide wire 90/10 Na-hep releases approximately 150-200 mU active heparin per cm coil within the first few minutes after incubation in buffer. The PTFE BAK-hep shows a relatively slow release of 60-70 mU active heparin per cm coil. The 90/10 BAK-hep showed no released heparin but the most exposed heparin. In a static experiment with human full blood excessive thrombus formation occurred at the heparin-free models, whereas the others remained essentially clean. In a thrombin-generation assay under flow the authors observed strong retardation of thrombin formation in the case of the 90/10 Na-hep guide wire.
Conclusions: The static and dynamic in vitro assays, taken together, show that the 90/10 Na-hep provides a coating with an extremely low level of surface thrombogenicity. Use of a guide wire with a hydrophilic distal coating that releases and exposes sodium heparin may contribute to the safety of diagnostic and therapeutic interventional procedures.