Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). In addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-beta-fructofuranosidase reaction rate were studied using a 2(2) factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V (t)/V (h) ratio. At 40 and 50 degrees C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes.