Genetic variation in the number of 21-d opportunity periods required to achieve pregnancy after the voluntary waiting period (VWP) had passed was examined using 44,901 lactation records of 29,422 lactating Holstein cows on 61 large commercial dairy farms in the United States. Cows were allowed a maximum of 8 opportunity periods, and the cumulative percentages of cows that became pregnant by the end of the first, second, third, fourth, and fifth opportunity periods were 19, 29, 37, 43, and 47%, respectively. In addition, 38% of records were censored because of culling or failure to achieve pregnancy after 8 opportunity periods. Mean days open was 128 d for complete records, whereas mean days to last service was 148 d for censored records. An ordinal censored threshold model was developed, in which duration of the VWP was estimated simultaneously with prediction of sire breeding values. The posterior mean of intraherd-year heritability for the number of 21-d opportunity periods required to achieve pregnancy was 0.06, with a posterior standard deviation of 0.01. Posterior means for duration of the VWP ranged from 28 to 74 d postpartum among the 116 herd-parity classes represented in the study, whereas farmer-reported survey values for duration of the VWP ranged from 30 to 78 d postpartum. Sires' predicted transmitting abilities were computed, assuming an unknown VWP (i.e., estimated from the data), a VWP fixed at 60 d postpartum, or a VWP fixed at farmer survey values. Correlations among sire predicted transmitting abilities from different models were > or = 0.98, although some reranking occurred among top sires. In summary, the proposed model for genetic evaluation of female fertility can accommodate heterogeneity in duration of the VWP between herds, as well as heterogeneity that may arise within herds owing to management practices such as intentional delay of first insemination in high-producing cows or cows with poor body condition, and it can also accommodate censored records for nonpregnant cows.