A trinuclear copper(II) complex of 2,4,6-tris(di-2-pyridylamine)-1,3,5-triazine shows prominent DNA cleavage activity

Inorg Chem. 2007 Apr 16;46(8):3306-12. doi: 10.1021/ic0614162. Epub 2007 Mar 14.

Abstract

A highly water soluble 3:2 complex of copper(II) and 2,4,6-tris(di-2-pyridylamine)-1,3,5-triazine (TDAT) has been synthesized and structurally characterized. The complex crystallized in a triclinic P1 space group with a molecular formula of [Cu3(TDAT)2Cl3]Cl3.2H2O (1), where each copper ion is coordinated by four pyridine nitrogen atoms and an apical chloride. The trinuclear complex is stable at physiological relevant conditions. It can bind to DNA through electrostatic attraction and cleave efficiently the supercoiled pBR322 DNA into its nicked and linear forms at micromolar concentrations. Active oxygen intermediates such as hydroxyl radicals and singlet oxygen generated in the presence of 1 may act as active species for the DNA scission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopyridines / chemistry*
  • Copper / chemistry*
  • Copper / metabolism
  • Crystallography, X-Ray
  • DNA / chemistry*
  • DNA / metabolism
  • Models, Molecular
  • Molecular Conformation
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Organometallic Compounds / metabolism
  • Oxidation-Reduction
  • Reactive Oxygen Species / chemistry
  • Triazines / chemistry*

Substances

  • 2,4,6-tris(di-2-pyridylamine)-1,3,5-triazine
  • Aminopyridines
  • Organometallic Compounds
  • Reactive Oxygen Species
  • Triazines
  • Copper
  • DNA