Oral administration of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 stimulates the gene expression of Na(+)-K(+)-2Cl- co-transporter 1 in rat ileal epithelial cells

Biosci Biotechnol Biochem. 2007 Mar;71(3):807-10. doi: 10.1271/bbb.60589. Epub 2007 Mar 7.

Abstract

The effect of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) on the gene expression of Na(+)-K(+)-2Cl(-) co-transporter 1 (NKCC1) in intestinal epithelial cells was evaluated by using rats. The NKCC1 gene in ileal epithelial cells was significantly up-regulated by the oral administration of EC-12. The results of this study suggest in vivo that EC-12 had the potential to stimulate intestinal NKCC1 expression.

MeSH terms

  • Administration, Oral
  • Animals
  • Enterococcus faecalis*
  • Epithelial Cells / metabolism*
  • Hot Temperature*
  • Ileum / metabolism*
  • In Vitro Techniques
  • Intestinal Mucosa / metabolism*
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Sodium-Potassium-Chloride Symporters / biosynthesis*
  • Solute Carrier Family 12, Member 2

Substances

  • Slc12a2 protein, rat
  • Sodium-Potassium-Chloride Symporters
  • Solute Carrier Family 12, Member 2