Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: a linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio

Haematologica. 2007 Mar;92(3):429-30. doi: 10.3324/haematol.10632.

Abstract

In order to overcome the problem of different control genes for BCR-ABL normalization, we used a linear regression equation to compare our results previously obtained using B2M as the control gene with those calculated using the ABL gene and validated the slope as a factor to convert from B2M to ABL results.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Computer Systems
  • Fusion Proteins, bcr-abl / biosynthesis*
  • Fusion Proteins, bcr-abl / genetics
  • Genes, abl
  • Humans
  • Linear Models
  • Polymerase Chain Reaction / methods*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / blood*
  • RNA, Messenger / genetics
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / blood*
  • RNA, Neoplasm / genetics
  • beta 2-Microglobulin / genetics

Substances

  • RNA, Messenger
  • RNA, Neoplasm
  • beta 2-Microglobulin
  • Fusion Proteins, bcr-abl