Thiols in nitric oxide synthase-containing Nocardia sp. strain NRRL 5646

Appl Environ Microbiol. 2007 May;73(9):3095-7. doi: 10.1128/AEM.02809-06. Epub 2007 Mar 2.

Abstract

Mycothiol (MSH) [1-D-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-D-glucopyranoside], isolated as the bimane derivative, was established to be the major thiol in Nocardia sp. strain NRRL 5646, a species most closely related to Nocardia brasiliensis strain DSM 43758(T). Thiol formation and detection of MSH-dependent formaldehyde dehydrogenase activity in cell extracts are relevant to the possible modulation of nitric oxide toxicity generated by strain NRRL 5646.

Publication types

  • Comparative Study

MeSH terms

  • Aldehyde Oxidoreductases / metabolism
  • Base Sequence
  • Chromatography, High Pressure Liquid
  • Cysteine / analysis
  • Cysteine / isolation & purification*
  • Glycopeptides / analysis
  • Glycopeptides / isolation & purification*
  • Inositol / analysis
  • Inositol / isolation & purification*
  • Molecular Sequence Data
  • Molecular Structure
  • Nitric Oxide / metabolism
  • Nocardia / chemistry*
  • Nocardia / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Glycopeptides
  • RNA, Ribosomal, 16S
  • mycothiol
  • Nitric Oxide
  • Inositol
  • Aldehyde Oxidoreductases
  • formaldehyde dehydrogenase, glutathione-independent
  • Cysteine

Associated data

  • GENBANK/DQ925490