Abstract
Pseudomonas putida DTB grew aerobically with N,N-diethyl-m-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and meta ring cleavage. A gene from DTB, dthA, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Biodegradation, Environmental
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Cloning, Molecular
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Computational Biology
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DEET / metabolism*
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DNA Primers
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Hydrolases / genetics*
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Hydrolases / metabolism
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Molecular Sequence Data
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Molecular Structure
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New York
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Pseudomonas putida / genetics
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Pseudomonas putida / metabolism*
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Sequence Alignment
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Sequence Analysis, DNA
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Sewage / microbiology
Substances
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DNA Primers
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Sewage
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DEET
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Hydrolases
Associated data
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GENBANK/EF123069
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GENBANK/EF123070