Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays

Vet Immunol Immunopathol. 2007 May 15;117(1-2):17-25. doi: 10.1016/j.vetimm.2007.01.022. Epub 2007 Feb 12.

Abstract

A gold-immunochromatographic test-strip kit is used for the detection of IgG antibodies against the nucleocapsid protein of Avian Influenza Virus (AIV). Compared with the "gold standard", i.e. hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) assays, the gold-immunochromatographic test strip has many advantages, such as high specificity, high sensitivity, convenience, is rapid and has low cost. The gold-immunochromatographic test strip provides a unique tool for the on-site surveillance and diagnosis of Avian Influenza.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Chickens*
  • Gold Colloid / chemistry
  • Hemagglutination Inhibition Tests / methods
  • Hemagglutination Inhibition Tests / veterinary
  • Immunodiffusion / methods
  • Immunodiffusion / veterinary
  • Immunoglobulin G / blood
  • Immunohistochemistry / methods
  • Immunohistochemistry / standards
  • Immunohistochemistry / veterinary*
  • Influenza A virus / immunology*
  • Influenza A virus / isolation & purification
  • Influenza in Birds / diagnosis
  • Influenza in Birds / immunology*
  • Influenza in Birds / virology
  • Nucleocapsid Proteins / immunology
  • Reagent Strips
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Specific Pathogen-Free Organisms

Substances

  • Antibodies, Viral
  • Gold Colloid
  • Immunoglobulin G
  • Nucleocapsid Proteins
  • Reagent Strips