Bioactive compounds from liverworts: Inhibition of lipopolysaccharide-induced inducible NOS mRNA in RAW 264.7 cells by herbertenoids and cuparenoids

Liva Harinantenaina; Dang Ngoc Quang; Takashi Nishizawa; Toshihiro Hashimoto; Chie Kohchi; Gen-Ichiro Soma; Yoshinori Asakawa

doi:10.1016/j.phymed.2006.09.011

Bioactive compounds from liverworts: Inhibition of lipopolysaccharide-induced inducible NOS mRNA in RAW 264.7 cells by herbertenoids and cuparenoids

Phytomedicine. 2007 Aug;14(7-8):486-91. doi: 10.1016/j.phymed.2006.09.011. Epub 2007 Mar 1.

Authors

Liva Harinantenaina¹, Dang Ngoc Quang, Takashi Nishizawa, Toshihiro Hashimoto, Chie Kohchi, Gen-Ichiro Soma, Yoshinori Asakawa

Affiliation

¹ Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan.

PMID: 17336048
DOI: 10.1016/j.phymed.2006.09.011

Abstract

The inhibition of lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) by herbertenoids and cuparenoids isolated from liverworts in RAW 264.7 macrophages was evaluated. Among compounds tested, herbertenediol, cuparenediol, 1,2-diacetoxyherbertene and 2-hydroxy-4-methoxycuparene exhibited significant activity. For 2-hydroxy-4-methoxycuparene, chosen as representative compound, the strong inhibitory activity was related to the inhibition on LPS-induced iNOS mRNA. The structure-activity relationship will be discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Hepatophyta / chemistry*
Lipopolysaccharides / pharmacology*
Macrophages / drug effects
Mice
Molecular Structure
Nitric Oxide / metabolism
Nitric Oxide Synthase Type II / metabolism*
Plant Extracts / chemistry
Plant Extracts / pharmacology*
RNA, Messenger / metabolism
Sesquiterpenes / chemistry
Sesquiterpenes / pharmacology*

Substances

Lipopolysaccharides
Plant Extracts
RNA, Messenger
Sesquiterpenes
Nitric Oxide
Nitric Oxide Synthase Type II