Detection of pesticide by polymeric enzyme electrodes

K Dutta; D Bhattacharyay; A Mukherjee; S J Setford; A P F Turner; P Sarkar

doi:10.1016/j.ecoenv.2007.01.004

Detection of pesticide by polymeric enzyme electrodes

Ecotoxicol Environ Saf. 2008 Mar;69(3):556-61. doi: 10.1016/j.ecoenv.2007.01.004. Epub 2007 Feb 27.

Authors

K Dutta¹, D Bhattacharyay, A Mukherjee, S J Setford, A P F Turner, P Sarkar

Affiliation

¹ Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, Kolkata 700009, India.

PMID: 17328951
DOI: 10.1016/j.ecoenv.2007.01.004

Abstract

Screen-printed electrodes (SPEs) containing immobilized acetylcholine esterase (AChE) enzyme were used for the electrochemical determination of organophosphorous (OP) and carbamate pesticides. The extent of AChE deactivation by the pesticide was determined in the presence of acetylcholine (AChCl) substrate. The unique nature of this approach lies in the enzyme immobilization procedure in which AChE was attached to the SPE by in situ bulk polymerization of acrylamide to ensure efficient adherence within the membrane with minimal losses in enzyme activity. Responses were observed for the pesticides Monocrotophos, Malathion, Metasystox and Lannate over the concentration range 0-10 ppb (microg L(-1)).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylcholinesterase / metabolism*
Carbamates / toxicity
Cholinesterase Inhibitors / analysis
Electrochemistry / methods
Electrodes
Enzymes, Immobilized / antagonists & inhibitors*
Hydrogen-Ion Concentration
Kinetics
Pesticides / analysis*
Pesticides / toxicity

Substances

Carbamates
Cholinesterase Inhibitors
Enzymes, Immobilized
Pesticides
Acetylcholinesterase

Grants and funding

064323/Wellcome Trust/United Kingdom