Y-chromosomal STRs loci were analyzed from a sample of 355 healthy unrelated male individuals of South Korean population. Allele and haplotype frequencies for DYS441, DYS442, DYS443, DYS444, DYS445, DYS446, DYS452 and DYS456 were determined by the general PCR and silver staining methods. The gene diversity values for the Y-STRs loci ranged from 0.4902 (DYS465) to 0.7883 (DYS446). A total of 263 haplotypes were identified in the Y-STR loci, among which 225 were unique, while 38 occurred more than once. And the combined haplotypes diversity was 0.9958.