Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences

Dong Suk Park; Jae Kyung Shim; Jung Sun Kim; Chun Keun Lim; Rosemary Shrestha; Jang Ho Hahn; Hong Gi Kim

doi:10.1016/j.micres.2006.11.005

Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences

Microbiol Res. 2009;164(1):36-42. doi: 10.1016/j.micres.2006.11.005. Epub 2007 Feb 21.

Authors

Dong Suk Park¹, Jae Kyung Shim, Jung Sun Kim, Chun Keun Lim, Rosemary Shrestha, Jang Ho Hahn, Hong Gi Kim

Affiliation

¹ National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea. dspark@rda.go.kr

PMID: 17317128
DOI: 10.1016/j.micres.2006.11.005

Abstract

The present study describes PCR assay to detect bacterial spot caused by Xanthomonas campestris pv. vesicatoria in pepper and tomato. One set of PCR primer was developed to amplify gene required for an rhs family gene homologous to rhsA, cell envelope biogenesis, outer membrane. Only a PCR product of a 517bp was produced in PCR reaction with the Xanthomonas campestris pv. vesicatoria (XCVF/XCVR) primer set. A specific, and highly sensitive and rapid PCR assay for the detection of X. campestris pv. vesicatoria was achieved. The protocol can be used as a reliable diagnostic tool for specific detection of X. campestris pv. vesicatoria in pepper or tomato.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics*
Capsicum / microbiology
DNA Primers / genetics*
Multigene Family*
Plant Diseases / microbiology*
Polymerase Chain Reaction / methods*
Solanum lycopersicum / microbiology
Species Specificity
Xanthomonas campestris / genetics
Xanthomonas campestris / isolation & purification*

Substances

Bacterial Proteins
DNA Primers