Real-time monitoring of restriction endonuclease activity using molecular beacon

Anal Biochem. 2007 Apr 15;363(2):294-6. doi: 10.1016/j.ab.2007.01.018. Epub 2007 Jan 19.

Authors

Changbei Ma¹, Zhiwen Tang, Kemin Wang, Weihong Tan, Xiaohai Yang, Wei Li, Zhaohui Li, Xiaoyuan Lv

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Hunan University, Changsha 410082, People's Republic of China.

PMID: 17313935
DOI: 10.1016/j.ab.2007.01.018

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry
DNA / metabolism
DNA Restriction Enzymes / analysis
DNA Restriction Enzymes / metabolism*
DNA-Directed DNA Polymerase / metabolism
Deoxyribonucleases, Type II Site-Specific / analysis
Deoxyribonucleases, Type II Site-Specific / metabolism
Enzyme Activation / drug effects
Heparin / pharmacology
Nucleic Acid Hybridization / methods
Oligonucleotide Probes / chemistry
Oligonucleotide Probes / genetics*
Reproducibility of Results

Substances

Oligonucleotide Probes
Heparin
DNA
DNA-Directed DNA Polymerase
DNA Restriction Enzymes
Deoxyribonucleases, Type II Site-Specific
GTAC-specific type II deoxyribonucleases