HPLC and HPLC/MS are the most widely used analytical techniques in the field of pesticides analysis. In recent years, there has been considerable focus on fast separations in HPLC in order to reduce analysis time as well as cost. Monolithic columns, consisting of continuous beds with macropores and mesopores, can meet this requirement and have been widely used in the medical and biological fields. However, it has seldom been used when analyzing pesticides. In this work, the application of monolithic columns in pesticides analysis and their advantages are evaluated and compared with those obtained using conventional packed columns.