Abstract
The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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4-Butyrolactone / metabolism*
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Agrobacterium tumefaciens / enzymology
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Agrobacterium tumefaciens / genetics
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Agrobacterium tumefaciens / metabolism*
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Carboxylic Ester Hydrolases / genetics
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Carboxylic Ester Hydrolases / metabolism*
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DNA Footprinting
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DNA, Bacterial / metabolism
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Electrophoretic Mobility Shift Assay
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Gene Deletion
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Models, Biological
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Oxidoreductases / genetics
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Oxidoreductases / metabolism*
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Repressor Proteins / physiology*
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Succinic Acid / metabolism*
Substances
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DNA, Bacterial
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Repressor Proteins
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Succinic Acid
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Oxidoreductases
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Carboxylic Ester Hydrolases
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N-acyl homoserine lactonase
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4-Butyrolactone