Peroxidation of mineral oil used in droplet culture is detrimental to fertilization and embryo development

Junko Otsuki; Yasushi Nagai; Kazuyoshi Chiba

doi:10.1016/j.fertnstert.2006.11.144

Peroxidation of mineral oil used in droplet culture is detrimental to fertilization and embryo development

Fertil Steril. 2007 Sep;88(3):741-3. doi: 10.1016/j.fertnstert.2006.11.144. Epub 2007 Feb 12.

Authors

Junko Otsuki¹, Yasushi Nagai, Kazuyoshi Chiba

Affiliation

¹ Department of Biology, Ochanomizu University, Tokyo, Japan. midori@nagai-cl.com <midori@nagai-cl.com>

PMID: 17292894
DOI: 10.1016/j.fertnstert.2006.11.144

Abstract

Unexpected deterioration of human embryos led us to an investigation of possible toxic factors in the oil covering culture medium. Elevated levels of peroxidation in the oil by oxygen exposure or unfavorable storage were found to be the main causes of toxicity.

MeSH terms

Blastocyst / drug effects
Blastocyst / pathology
Cell Culture Techniques
Embryonic Development*
Female
Fertilization / drug effects
Fertilization / physiology*
Fertilization in Vitro
Humans
Lipid Peroxidation* / drug effects
Mineral Oil / toxicity*
Pregnancy

Substances

Mineral Oil