Abstract
The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gave rise to ribostamycin production in various culture broths. This is the first report of heterologous aminoglycoside production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoglycosides / chemistry*
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Anti-Bacterial Agents / chemical synthesis*
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Biochemistry / methods
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Chemistry, Pharmaceutical / methods*
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Cosmids
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Drug Design
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Models, Chemical
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Molecular Conformation
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Plasmids / metabolism
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Ribostamycin / chemistry*
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Spectrometry, Mass, Electrospray Ionization
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Streptomyces lividans / metabolism*
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Ribostamycin