On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35

Rimantas Daugelavicius; Ausra Gaidelyte; Virginija Cvirkaite-Krupovic; Dennis H Bamford

doi:10.1016/j.mimet.2006.12.023

On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35

J Microbiol Methods. 2007 Apr;69(1):174-9. doi: 10.1016/j.mimet.2006.12.023. Epub 2007 Jan 16.

Authors

Rimantas Daugelavicius¹, Ausra Gaidelyte, Virginija Cvirkaite-Krupovic, Dennis H Bamford

Affiliation

¹ Department of Biological and Environmental Sciences and Institute of Biotechnology, University of Helsinki, Finland.

PMID: 17289190
DOI: 10.1016/j.mimet.2006.12.023

Abstract

In this study an on-line electrochemical method was developed to examine the one-step growth cycle (OSGC) of the bacteriophage Bam35. The on-line conditions for monitoring the OSGC and the effect of aeration on the duration of the OSGC were defined. The data indicate that binding of phenyldicarbaundecaborane anions to Bacillus thuringiensis cells infected with Bam35 can be used as a sensitive indicator of cell lysis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacillus Phages / growth & development*
Bacillus thuringiensis / metabolism
Bacillus thuringiensis / virology*
Boron Compounds / metabolism
Cell Culture Techniques
Lysogeny / physiology*
Time Factors

Substances

Boron Compounds
phenyldicarbaundecaborane