[Interaction investigation of trypsin inhibitor from sea anemone Radianthus macrodactylus with proteases]

I N Sokotun; O V Gnedenko; E V Leĭchenko; M M Monastyrnaia; E P Kozlovskaia; A A Mol'nar; A C Ivanov

[Interaction investigation of trypsin inhibitor from sea anemone Radianthus macrodactylus with proteases]

Biomed Khim. 2006 Nov-Dec;52(6):595-600.

[Article in Russian]

Authors

I N Sokotun, O V Gnedenko, E V Leĭchenko, M M Monastyrnaia, E P Kozlovskaia, A A Mol'nar, A C Ivanov

PMID: 17288251

Abstract

The interaction of inhibitor VJ (InhVJ), isolated from sea anemone R. macrodactylus, with different proteases was investigated. The following enzymes were tested: serine proteases (trypsin, alpha-chymotrypsin, plasmin, thrombin, kallikrein), cysteine protease (papain) and aspartic protease (pepsin). Inhibitor VJ interacted only with trypsin and 6-chymotrypsin. Kinetic and thermodynamics parameters of intermolecular complexes formation were determined: KD = 7,38 x 10(-8) M and 9,93 x 10(-7) M for pairs InhVJ/trypsin and InhVJ/alpha-chymotrypsin, respectively.

Publication types

Comparative Study
English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Kinetics
Peptide Hydrolases / chemistry*
Peptide Hydrolases / metabolism
Protein Binding
Sea Anemones / chemistry*
Sea Anemones / metabolism
Trypsin Inhibitors / chemistry*
Trypsin Inhibitors / isolation & purification
Trypsin Inhibitors / metabolism

Substances

Trypsin Inhibitors
Peptide Hydrolases