Evaluation of VITEK 2 and RapID yeast plus systems for yeast species identification: experience at a large clinical microbiology laboratory

Maurizio Sanguinetti; Rosaria Porta; Michela Sali; Marilena La Sorda; Giovanni Pecorini; Giovanni Fadda; Brunella Posteraro

doi:10.1128/JCM.02469-06

Evaluation of VITEK 2 and RapID yeast plus systems for yeast species identification: experience at a large clinical microbiology laboratory

J Clin Microbiol. 2007 Apr;45(4):1343-6. doi: 10.1128/JCM.02469-06. Epub 2007 Feb 7.

Authors

Maurizio Sanguinetti¹, Rosaria Porta, Michela Sali, Marilena La Sorda, Giovanni Pecorini, Giovanni Fadda, Brunella Posteraro

Affiliation

¹ Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito, 1-00168 Rome, Italy. msanguinetti@rm.unicatt.it

Abstract

A total of 750 clinical yeast isolates were evaluated by two identification systems, VITEK 2 and RapID Yeast Plus, using sequence analysis of the rRNA gene internal transcribed spacer regions as the reference method. The VITEK 2 and RapID systems correctly identified 737 (98.2%) and 716 (95.5%) isolates, respectively.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA, Fungal / chemistry
DNA, Fungal / genetics
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Humans
Mycology / methods*
Mycoses / microbiology
Sequence Analysis, DNA
Yeasts / classification*
Yeasts / isolation & purification*

Substances

DNA, Fungal
DNA, Ribosomal Spacer