[Influence of maternal age on meiotic spindle and chromosome configuration of oocytes]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Feb;24(1):6-9.
[Article in Chinese]

Abstract

Objective: To study the effect of maternal age on meiotic spindle and chromosome configuration of oocytes.

Methods: Spindle and chromosome configuration was examined in day 1 unfertilized human oocytes after in vitro fertilization (IVF) and intracytoplasmic sperm injection(ICSI) by immunocytochemistry and visualized by laser confocal microscopy.

Results: Statistically significant differences were observed on normal spindle and chromosome configurations of oocytes between 25-29 maternal age group (33% and 31%, respectively), and 30-34 age group (P< 0.05) as well as 35-40 age group(0%, P<0.01). The incidence of abnormal spindle and chromosome configurations of oocytes from 30-34 and 35-40 maternal age groups was much higher than that of oocytes from 25-29 age group (P<0.01, P<0.05).

Conclusion: Incidence of abnormal spindle and chromosome configuration of oocytes is related to maternal age. It could be an important reason of age related oocyte aneuploidy.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Age Factors
  • Chromosomes, Human / metabolism*
  • Female
  • Fertilization in Vitro
  • Humans
  • Immunohistochemistry
  • Microscopy, Confocal
  • Oocytes / metabolism*
  • Sperm Injections, Intracytoplasmic
  • Spindle Apparatus / metabolism*